Universal microarrays for the evaluation of fresh-.. (ΜΑQUΑ)
Universal microarrays for the evaluation of fresh-water quality based on detection of pathogens and their toxins
Date du début: 1 mars 2011,
Date de fin: 30 nov. 2014
Monitoring the quality of drinking water is of paramount importance for public health. “Water is not a commercial product but a heritage that must be protected, defended and treated as such” (Water Framework Directive 2000/60/EC). The threat of waterborne diseases in Europe will predictably increase in the future as the human population increases and as a result of globalization and migration from non-EU countries and of climate change. Development of efficient, sensitive, robust, rapid and inexpensive tests to monitor various aspects of water quality represents an essential milestone within the strategy for control and prevention of diseases caused by waterborne pathogens and by algal toxins. Traditional methods for the detection of waterborne pathogens, based on cultivation, biochemical characterisation and microscopic detection are laborious and time-consuming; molecular biological tools have now greatly enhanced our ability to investigate biodiversity by identifying species and to estimate gene flow and distribution of species in time and space. μAQUA aims to design and develop a universal microarray chip for the high-throughput detection in water of known and emerging pathogens (bacteria, viruses, protozoa and cyanobacteria) and to assess the water quality monitoring the presence of select bioindicators (i.e. diatoms). A chip able to detect cyanobacterial toxins will also be developed. These innovative molecular tools should be amenable to automation so that they could be deployed on moorings for routine semi-continuous monitoring of water quality. μAQUA also aims to identify cyanophages potentially capable of controlling and mitigating the periodical blooming of toxic cyanobacteria in drinking water reservoirs. Overall, these innovative and cost efficient technologies will reduce energy requirements and improve performance of water treatment, and allow rapid management response to new situations brought about by environmental (including climatic) changes.
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