Structure-Function Studies of the TRIM Family of U.. (TRIM)
Structure-Function Studies of the TRIM Family of Ubiquitin E3 Ligases
Date du début: 1 juin 2014,
Date de fin: 31 mai 2018
"Ubiquitin E3 enzymes participate in the last step of a three-enzyme cascade, where they transfer ubiquitin from E2 enzymes to substrates, therefore are responsible to bring the right substrate to the right E2 enzyme. The tripartite motif (TRIM) family of ubiquitin E3 ligases includes 70 members that are involved in cellular processes such as immune signaling, transcriptional regulation and apoptosis. Defects in TRIM E3 ligase activity have been linked to cancer and developmental and genetic diseases. Despite TRIM vital roles, important aspects of their mechanism as E3 ligases remain unknown mainly due to their multi-domain architecture, their ability to self-associate into dimers and higher order complexes, and their ability to serve as E3 ligases not only for ubiquitin but also for ubiquitin-like proteins. We will pursue our interest in the molecular mechanism of TRIM E3 activity by working on TRIM25 that possesses dual E3 activity for ubiquitin and ubiquitin-like protein ISG15. We will determine the molecular mechanism of TRIM25 E3 activity using molecular biology, cell biology, crystallography and biochemistry. Specifically, we will map the contribution of TRIM25 different domains and the oligomineric state to its E3 activity. Then, together with crystal structures of TRIM25 with its cognate E2, UBCH8, charged with ubiquitin or ISG15, we will provide structural insight on how TRIM25 works in concert with UBCH8 and possesses dual E3 activity. In addition, we will determine how TRIM25 interacts with its substrate, 14-3-3σ, and whether the oligomeric state is important for substrate binding. Since 14-3-3σ undergoes ubiquitination and ISGylation by TRIM25 we will investigate the potential crosstalk between modifications. Specifically, we will test for the formation of mixed ubiquitin-ISG15 chains. Our work will provide mechanistic details on TRIM E3 ligase activity as well as critical data for future research on TRIM proteins as targets for therapeutic drug design."
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