"Since the histopathological diagnosis of tumours is based on microscopy, it would be highly desirable to possess a microscopical technique allowing morphological investigation with a wide zoom range, high resolution and the implementation of multiplex staining. Thus, the goal of the REMEDI project is to explore the possibilites of novel light microscopy techniques, which overcome the diffraction limit of visible light and extend the range of observation to the molecular level of proteins in tumour cells and analyze their interaction and spatial distribution. This proposal aims at the use of single-molecule-microscopy of cancer tissue samples with ultra-high sensitivity, simplified handling and increased speed of analysis. This shall be accomplished by the development of a novel resolution-enhanced microscopy platform with an integrated novel CMOS camera and an adequate image acquistion and analysis software. The REMEDI platform will be validated with two relevant applications: diagnostic/experimental pathology of breast cancer and alterations of plasma membrane components of lymphoma cells."