The applicant has previously worked and published in the area of cell migration and took a career break for family reasons. She now wishes to pursue a career in immunology with a particular focus the study of T cell migration. The host laboratory in Trinity College Dublin (TCD) will providethe applicant with the ideal opportunity to develop essential practical and theoretical skills in this field of research. The host scientist, Dr Aideen Long, has worked in this area for more than 10 years with a considerable track record and high impact publications. Her group has identified threenovel proteins that demonstrate a polarized distribution in migrating T cells. The scientific aim of the proposed project is to investigate the functional role of these novel proteins - Synaptopodin, Spectrin beta 1 and Neurofilament Heavy chain in T cell polarization and migration. This will train the Fellow in the use of cutting edge technologies such as proteomics, siRNA and High Content Analysis - a revolutionary technology, which combines automated fluorescence microscopy with multi-parametric analysis. As TCD's expertise in immunology is recognised globally, the research environment and proposed project together with complementary skills training will enhance the fellow's education and professional development. It will allow her both to re-establish her scientific career and to return to Poland with important additional research expertise.