The objective of this project is the integration of world class high-throughput sequencing and genotyping facilities that will provide sequencing and genotyping technologies and data analysis methodologies to the scientific community.The European Sequencing and Genotyping Infrastructure (ESGI) will enable external users to generate data rapidly and to acquire knowledge efficiently. By providing access to the ESGI facilities in order to benefit from the sequencing and genotyping technologies, there will be an outreach and sustainable impact for the scientific community in the area of biological and medical research to generate new knowledge.The ESGI will optimise European research programs and foster transnational collaborations. In general, the ESGI will defragment and thereby strengthen the European research capacities in genetics and genomics and improve the knowledge transfer from large genomics centers among themselves and to external expert groups or scientists who are focusing on specific research questions.Our aim is to apply and improve new high-throughput nucleic acids analysis technologies for a broad range of genetic and systems biology studies using well-phenotyped samples, for example those derived from standardised European biobanks and animal facilities. In particular, massively-parallel sequencing technologies are essential components of modern biomedical research and are ready to reveal molecular and cellular pathways underlying complex traits and common diseases.As the European Strategy Forum for Research Infrastructures (ESFRI) pointed out in the past, the development of an efficient infrastructure for sequencing and genotyping is of crucial importance to position Europe as one of the world-leading regions for genetics, genomics and systems biology research and thus a contribution to the European Research Area.