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Capturing kinase-substrate pairs in intact mammalian cells by using unnatural amino acid mutagenesis and photocrosslinking (Kinase crosslinking)
Date du début: 1 févr. 2011, Date de fin: 31 janv. 2014,
Aim of this project is to develop a method for capturing kinase-substrate pairs in living mammalian cells using unnatural amino acid mutagenesis and affinity crosslinking. Photocrosslinking amino acids (containing benzophenone or azide groups) will be introduced site-specifically into the backbone of target proteins using ad hoc developed orthogonal tRNA/synthetase pairs functional in mammalian ce ...
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