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Local temperature jumps for single Protein Molecules: a route to study dynamics from Microseconds to hours (LTJSPM)
Date du début: 1 août 2007, Date de fin: 31 juil. 2009,
We propose to physically trap a single bio-molecule into successive conformational sub-states by rapidly freezing and thawing it with its immediate surroundings. After many successive freeze-thaw cycles, we will reconstruct the dynamics of the same single molecule from the series of frozen snapshots, probed by the fluorescence of optical labels. Fast cooling and heating will be achieved by maintai ...
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