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Development of novel multiplexed SPEX-based methodologies to accurately genotype forensic, archaeological, and other marginal environmental sources of DNA (Multiplexed-SPEX)
Date du début: 1 janv. 2009, Date de fin: 31 déc. 2010,
Traces of surviving degraded DNA in marginal clinical, forensic, archaeological, and environmental samples are generally amplified by PCR, which can often produce highly-skewed product distributions dominated by non-authentic sequences (due to DNA damage and PCR-generated artefacts). The applicant has developed the SPEX amplification methodology, an alternative to PCR which has generated sequence ...
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